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- Posts: 15
- Joined: Sun Oct 04, 2009 10:54 pm
Recently, I came across a problem of cleaning up sugar phosphate using oasis MAX. The protocol was developed based on Waters brochure. The sugar phosphate standards (included Ribose 1-5-p, Fructose 1-6-p, 6-phosphogluconic acid, ribose 5-p) was loaded on the cartridge at neutral pH and wash with H2O. I collected the fraction from loading and washing and didn't see any sugar phosphate, which meant MAX can trap the sugar-p very well. However, I couldn't elute them out well. I 've tried to make elution pH drop close to 0.2 by adding HCl and tried to use 70% ACN instead of 100% MeOH. But whatever I changed eluting condition, the best recovery I got was just around 55%.
Anyone have any idea to make sugar-p come out the cartridge. Can Oasis WAX work for them as sugar-p first pka is close to 1.0. Or any other SPE cartridge can suit better for sugar phosphate?
Thanks any commends to help me out!