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- Posts: 22
- Joined: Wed Jun 09, 2010 8:36 am
I have difficulties in analysing serum containing peptide (as analyte) and Dow Corning 9509 silicone elastomer as one of matrix components. The silicone and my analyte has same retention time, so I always get higher recovery. I use Acetonitrile : water gradient for my mobile phase.
I have tried to separate it, but it doesn't work until now. So I make a linearity of matrix, and calculate my analyte recovery manually. Am i allowed to do that?
Or maybe some of you have experience with silicone elastomer? Please reply me then. Thanks a lot.