Extracting a peak for UV/VIS Analysis

Discussions about sample preparation: extraction, cleanup, derivatization, etc.

6 posts Page 1 of 1
Does anyone know what is entailed in extracting a peak from a HPLC-UV chromatogram for UV-VIS analysis?
Not likely based on the information you've not provided...

Please add some specifics, like what data system you're using for starters.
Thanks,
DR
Image
Maybe this query will make more sense:

If you run a sample and see multiple peaks using LC-UV and want to extract one of the peaks for PDA analysis, how would you do that?
When working with PDA, you probably want to extract a wavelength chromatogram. If only one substance absorbs well on that particular wavelength, then you'll eventually have an extracted chromatogram with just one peak. But if other compounds also absorb at that wavelength, you'll still end up with multiple peaks.
Software Engineer at elsci.io (my contact: stanislav.bashkyrtsev@elsci.io)
sbashkyrtsev wrote:
When working with PDA, you probably want to extract a wavelength chromatogram. If only one substance absorbs well on that particular wavelength, then you'll eventually have an extracted chromatogram with just one peak. But if other compounds also absorb at that wavelength, you'll still end up with multiple peaks.


Please give a step by step on how to do this with lets say a LC-UV chromatogram with 2 peaks.
Mouleyre wrote:
Please give a step by step on how to do this with lets say a LC-UV chromatogram with 2 peaks.

1. Find the corresponding command for the chromatogram extraction in your software.
2. Choose the wavelength you need (from the recorded range of wavelengths) and use this command.
6 posts Page 1 of 1

Who is online

In total there is 1 user online :: 0 registered, 0 hidden and 1 guest (based on users active over the past 5 minutes)
Most users ever online was 1117 on Mon Jan 31, 2022 2:50 pm

Users browsing this forum: No registered users and 1 guest

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry