Liquid Liquid Extraction of Metformin

Discussions about sample preparation: extraction, cleanup, derivatization, etc.

2 posts Page 1 of 1
Hi All

Metformin is supplied as Metformin hydrochloride for solubility and I was wondering if it is possible to seperate Metformin base from the HCl by liquid liquid extraction?
pKa is 12.3, so if I raise the pH to 14, could I drive the Metformin out of the aqueous phase to seperate? Any feedback much appreciated.
Check out EPA Method 1694 https://www.epa.gov/sites/production/fi ... 4_2007.pdf

Metformin is listed in the Basic fraction and it has procedures for both acidic and basic drug extractions by solid phase extraction. They use the pH adjustment to 10 for the basic analytes. It would probably work similar if doing a liquid liquid extraction in a separatory funnel.
The past is there to guide us into the future, not to dwell in.
2 posts Page 1 of 1

Who is online

In total there is 1 user online :: 0 registered, 0 hidden and 1 guest (based on users active over the past 5 minutes)
Most users ever online was 1117 on Mon Jan 31, 2022 2:50 pm

Users browsing this forum: No registered users and 1 guest

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry