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- Posts: 6
- Joined: Thu Mar 11, 2021 6:43 pm
I am having trouble with my sample prep. I have a biologically derived antibiotic compound which we determine the presence of through inhibition assays. The compound is derived from a bacterial culture media. I have been trying to separate the active fraction using a C18 cartridge.
I am using a 1mL capacity cartridge with 100mg sorbent bed. 500uL of sample added results in the Load flow-through having the most activity, followed by the H2O wash and the solvent wash, respectively. Though there is variation, the differences are small, indicating the active compound is both passed-through and retained by the column. We know this by comparing the dilution sensitivity of our microbial inhibition assays for each collection.
What do you believe is occurring? Recommendations for other types of cartridges?
I am fairly new to this particular technique.
Thank you.