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- Posts: 13
- Joined: Thu Jul 16, 2009 2:29 pm
I am purifying peptide using TFA in Water/Acetonitrile. After lyophilization, the peptide is in TFA salt form. As I have to use the peptide for some cell experiment, I would like to change the peptide from TFA salt form to some cell friendly salt forms (Cl-, Acetate).
Someone told me I can dissolve peptide in 0.1M HCl and lyophilize to remove TFA. However I was also warned I may have to do the process several times to completely remove TFA, which I am afraid will result in loss of my peptides.
Did anyone do this before? What is the yield? Or could anyone give me some other suggestions?