Vancomycin from tissues

Discussions about sample preparation: extraction, cleanup, derivatization, etc.

2 posts Page 1 of 1
Dears
Does anyone have experience with Vancomycin (glycopeptide antibiotic) extraction from animal tissue?
The molecule is large (1450 Da) and highly polar. LC/MS/MS analytics is no problem with LOQ < 1 µg/L in water.
I tried extraction from tissues (liver, kidney and subcutaneous tissue) with the following methods:
1.) QuPPe-AO method => Recovery 0 %
2.) QuEChERs (buffered) => Recovery 0 %
3.) Homogenisation with excess water, centrifugation, filtration => Recovery < 10 %
3.) Homogenisation with lower amounts of methanol/water => Recovery < 10 %
4.) Homogenisation with metaphosphoric acid/methanol => Recovery < 5 %
5.) Homogenisation with water, cooking in microwave => Recovery 50 % (one-time lucky shot, sadly)
6.) Homogenisation with 0.3% metaphosphoric acid, cooking in microwave => Recovery < 20 %
6.) Homogenisation with less water, repeated cooking / resuspension of pellet in water bath at 80 °C => Recovery 20 - 30 %
7.) Addition of NaCl to 6. => Recovery 20 - 30 %

I tested for matrix effects => not significant, spiked blank extracts are OK.
I tested cooking stability => stable during mild cooking but not in the microwave

I have also tried to remove fat and debris with LLE (cyclohexane and dichloromethane) and dSPE (C18, PSA, diatomaceous earth) => no improvement

I hoped for literature because Vancomycin is an old molecule, but found only methods for plasma, urine and water.

Any further suggestions?
Thanks a lot
Jörg

PS.: I know from literature that Vancomycin is not metablised in liver, so I don't think that we see enzymatic degradation or similar biological effects.
No personal experience on vancomycin, but you might find some interesting information in this paper:

https://www.sciencedirect.com/science/a ... 4620321889
2 posts Page 1 of 1

Who is online

In total there is 1 user online :: 0 registered, 0 hidden and 1 guest (based on users active over the past 5 minutes)
Most users ever online was 1117 on Mon Jan 31, 2022 2:50 pm

Users browsing this forum: No registered users and 1 guest

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry