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- Posts: 418
- Joined: Thu Sep 25, 2008 3:40 am
in analyzing food sample, first homogenize sample , dope surrogates, then extract and finally clean-up. After cleaning up, we spike analytes into the final extract (for blank sample) and analyze by GC/MS or LC/MS/MS to create matrix-matched calibration (eg. 3 levels)
I have a question: why don't we spike analyte just before the extraction step to make calibration curve. I think that also covers the recovery and we do not need surrogates ?