SPE for Stevia
Posted: Tue Mar 22, 2022 4:50 pm
I am working on artificial sweeteners. Does anyone have a good SPE for steviol glycosides. I am trying to quantitate it in protein drinks and powders and carrez isn't sufficient. I am operating at UV210 and steviol glycosides have a low response with that so I need a really clean extract. Steviol glycosides give about 1/10 the signal of other sweeteners like aspartame, acesulfame.
Literature shows both normal phase silica SPE and C18 might work.
Literature shows both normal phase silica SPE and C18 might work.