SPE for Stevia

Discussions about methods and best practices across food quality, safety and authenticity testing.

2 posts Page 1 of 1
I am working on artificial sweeteners. Does anyone have a good SPE for steviol glycosides. I am trying to quantitate it in protein drinks and powders and carrez isn't sufficient. I am operating at UV210 and steviol glycosides have a low response with that so I need a really clean extract. Steviol glycosides give about 1/10 the signal of other sweeteners like aspartame, acesulfame.

Literature shows both normal phase silica SPE and C18 might work.
I tried a syringe c18 cartridge Macherey Nagel 731802 primed with methanol then water. I put 1ml sample onto it washed with 5ml water then eluted with 2 times 5ml of 80% acetonitrile. It reduced the blob matrix a fair amount but there was still a fair amount present coeluting.

There is a silica normal phase protocol but the sample needs to be in ethanol and then only 0.1ml can be applied and then eluted with 1ml so a 10x dilution plus the trouble of evaporating the sample first then possibly after the elution again.
2 posts Page 1 of 1

Who is online

In total there is 1 user online :: 0 registered, 0 hidden and 1 guest (based on users active over the past 5 minutes)
Most users ever online was 1117 on Mon Jan 31, 2022 2:50 pm

Users browsing this forum: No registered users and 1 guest

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.


Liquid Chromatography

Gas Chromatography

Mass Spectrometry