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I'm new to the chromatography world and my question must be very easy to solve... But I need some light!
So... I'm following a method in order to monitor some impurities during a stability study of a pharmaceutical product.
The method indicates few impurities, with their RRT, specification, among other information.
All of them are calculated based on the calibration curve obtained with the standard of the API, which is obtained at 254 nm..
But only 2 impurities must be quantified using a 210 nm wavelength...
My struggle is that I'm not able to use Empower in order to quantify these two impurities at 210 nm, using a calibration curve obtained at 254 nm...
I suppose I must create a different processing method for these 2 impurities and I've tried, but unsuccessfully.