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- Posts: 16
- Joined: Tue Jun 14, 2011 12:41 pm
System: Shiamdzu 20
Normal phase, heptane/IPA Mobile phases
typical normal phase column
1. I eliminated method/column issue by switching (IPA flush first) to Reverse phase (water/ACN/new C18 column/injected agilent isocratic std) split peaks still remain on early eluters
2. I changed out 100ul sample loop, HPV rotor seal and needle, plunger seal on LPV (metering device, essentially) split peaks still remains.
Typically split peaks are chemistry/solubility related, I cant seem to figure this one out, Any thoughts? thanks