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- Posts: 148
- Joined: Wed Jul 03, 2013 1:51 am
- Location: Michigan, USA
as many of you know, the separation of acrolein, acetone, and propanal DNPH derivatives is both challenging and important for certain applications. The standard methods are a reversed phase HPLC separation using C18 or phenyl-hexyl columns using water and acetonitrile as the mobile phases with typically 10-20% THF added to mobile phase 1.
Still my application note search found separations between the three critical peaks to be marginal and prone to causing problems down the line. In addition, I am not eager to use THF in the lab or have to resort to MS detection on a routine basis.
I have found an Agilent application note using acetone as mobile phase B that demonstrates very good separation between acrolein, acetone, and propanal. You can drive a truck between those peaks!
However, I am concerned about the mobile phase being a CARBONYL. Won't leftover unreacted DNPH react with the mobile phase upon injection and give a potentially very large and smeared out acetone-DNPH peak? Or am I thinking about something incorrectly?
Here is the application note in question: "Analysis of DNPH-derivatized Aldehydes and Ketones using the Agilent 1220 Infinity LC System with Diode Array Detector" Agilent. Sonja Schneider. 2016.
Thank you for your help in advance.
Arne