by
Mike H. » Tue Aug 27, 2019 7:31 pm
I made a method that separates 14 compounds, but I am having a problem with one of the compounds and I hope someone can give me ideas about how to approach it. The problem is that one of the imps is symmetrically broad with low plates where all the others are sharp with high plates. All compounds have a similar backbone with -COOH, amides and -OH substituents, but the broad peak has an aldehyde group and since this is the only difference from other compounds that have sharp peaks, it must be the cause.
Can someone list some potential cause for symmetrical broad peaks.
The gradient is straight forward 10mmol PPM, pH 2.5, ACN, 10% MeOH (constant. This is in method because I had 2 alcohol imps that fronted a lot early in the chromatogram. The inclusion improved the asymmetry from 0.7 to 1.1). Column is SB-Aq phase 100mm, 4.6mm x 1.8µm.
Any suggestions are welcomed. thx