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- Posts: 48
- Joined: Thu Jun 01, 2017 12:58 pm
- Location: Denmark
I was wondering if anyone has actual experience with that, as it sounds really dubious, and I can't really get my head around how you would even do it in practice, without either not getting all the analyte applied or getting huge amounts of air sucked into the system.
source: David Carr: A Guide to the Analysis and Purification of Proteins and Peptides by Reversed-Phase HPLC, p. 42, published by ACE columns
thanks in advance!
--Chris