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Purity by HPLC
HPLC System: Agilent 1100/1200 HPLC System
Flow Rate: 0.5 mL/min
Mobile Phase A: 0.04% TFA in Acetonitrile
Mobile Phase B: 0.04% TFA in Water
Column Temperature: 50°C ± 5°C
Autosampler Temperature: Ambient
Run Time: 16 minutes
Detection System: Shimazu ELSD-LT II
Temperature: 75°C
Gas Pressure: 50 psi
Detector Environment: 50HƵ
However, the %RSD for system suitability is out of 10%. Could anyone have an idea about solving the problem. Appreciate it!