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- Posts: 656
- Joined: Tue Jul 05, 2005 7:45 am
- Location: Denmark
I am running a lot of HILIC these days, but I have some problems with the columns. I have quite nice and pure samples, but on some columns I get peak distortion or peak splittning after only few injections. It seems to dependant on the individual column. I have columns that are still fine after many hundreds of injections.
I run only on silica columns (like Kinetex HILIC and Accucore HILIC). This is limiting the possibility to transfer the methods to QC.
Are HILIC columns harder to produce / more sensitive than standard C18 columns?