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HPLC-FLD baseline drift

Posted: Wed Jul 10, 2019 6:26 pm
by iris99
Hi,

I've been using a Agilent 1100 with a Dionex RF 2000 fluorescence detector with an ACN/H2O system and getting positive baseline drift with increasing %ACN (ex: 260nm, em: 350nm). This did not seem to happen in the past with the same procedure, and I've tried changing to fresh solvent and extending equilibration time, to no avail and the drift is repeatable between duplicate runs. Also of note, when I switched the ACN with MeOH to see if it was a solvent issue, the same spectra was observed.

I'm not sure what I can do to solve or narrow down the problem, any suggestions or comments would be much appreciated! Thanks!

Re: HPLC-FLD baseline drift

Posted: Wed Jul 10, 2019 8:47 pm
by James_Ball
iris99 wrote:
Hi,

I've been using a Agilent 1100 with a Dionex RF 2000 fluorescence detector with an ACN/H2O system and getting positive baseline drift with increasing %ACN (ex: 260nm, em: 350nm). This did not seem to happen in the past with the same procedure, and I've tried changing to fresh solvent and extending equilibration time, to no avail and the drift is repeatable between duplicate runs. Also of note, when I switched the ACN with MeOH to see if it was a solvent issue, the same spectra was observed.

I'm not sure what I can do to solve or narrow down the problem, any suggestions or comments would be much appreciated! Thanks!


Have you tried a new column? I have had FLD baseline problems when contaminates are highly retained on a column and bleed off slowly over time.

Re: HPLC-FLD baseline drift

Posted: Wed Jul 10, 2019 9:10 pm
by iris99
Hi there!

Yes this is with a new column (<1 month).