HPLC-FLD baseline drift

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

3 posts Page 1 of 1
Hi,

I've been using a Agilent 1100 with a Dionex RF 2000 fluorescence detector with an ACN/H2O system and getting positive baseline drift with increasing %ACN (ex: 260nm, em: 350nm). This did not seem to happen in the past with the same procedure, and I've tried changing to fresh solvent and extending equilibration time, to no avail and the drift is repeatable between duplicate runs. Also of note, when I switched the ACN with MeOH to see if it was a solvent issue, the same spectra was observed.

I'm not sure what I can do to solve or narrow down the problem, any suggestions or comments would be much appreciated! Thanks!
iris99 wrote:
Hi,

I've been using a Agilent 1100 with a Dionex RF 2000 fluorescence detector with an ACN/H2O system and getting positive baseline drift with increasing %ACN (ex: 260nm, em: 350nm). This did not seem to happen in the past with the same procedure, and I've tried changing to fresh solvent and extending equilibration time, to no avail and the drift is repeatable between duplicate runs. Also of note, when I switched the ACN with MeOH to see if it was a solvent issue, the same spectra was observed.

I'm not sure what I can do to solve or narrow down the problem, any suggestions or comments would be much appreciated! Thanks!


Have you tried a new column? I have had FLD baseline problems when contaminates are highly retained on a column and bleed off slowly over time.
The past is there to guide us into the future, not to dwell in.
Hi there!

Yes this is with a new column (<1 month).
3 posts Page 1 of 1

Who is online

In total there are 12 users online :: 1 registered, 0 hidden and 11 guests (based on users active over the past 5 minutes)
Most users ever online was 599 on Tue Sep 18, 2018 9:27 am

Users browsing this forum: Google [Bot] and 11 guests

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry