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My laboratory provides me with Agilent XDB-C18 column. Is it suitable to determine vitamin C in fruits (apple, orange)? What are pros and cons of this column in this case?
Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.
Bessie wrote:
Hi
My laboratory provides me with Agilent XDB-C18 column. Is it suitable to determine vitamin C in fruits (apple, orange)? What are pros and cons of this column in this case?
lmh wrote:
it's the extraction, not the chromatography, that's difficult. Extracting ascorbate without loss is very hard, and the common literature methods are actually rubbish, but no one dares admit it, because they've been around so long (sorry, feeling grumpy about this one).
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