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- Posts: 14
- Joined: Mon Jun 26, 2017 2:32 am
Recently we bought Atlantis, HILIC OBD prep 5 µm, Dimesion 19 mm× 150mm column for the purification of hydrophilic compounds.
The mobile phase mostly used in HILIC are 90/10 acetonitrile/ 100mM ammonium formate pH3.
My question is it necessary to use buffer ammonium formate or acetate to retain hydrophilic compounds, as my compound are sensitive to low pH.
Can I used only water and Acetonitrile to achieve good separation?
any suggestion will be highly appreciated.
Regards.