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- Posts: 6
- Joined: Tue Dec 02, 2008 7:47 pm
I read an article in which was described a method for HPLC detection of lipase activity.
Thy wrote:
[/quote] "Twenty microlitres of each supernatant obtained were injected onto a RP Supelcosil LC-18 column (5 μm; 4.6 × 250 mm). The amounts of products and residual substrate were determined from the peak areas of the HPLC-separated compounds with reference to appropriate standards. The elution of the samples occurred at 1.3 ml/min in 100 mM TFK, pH 6.0."
I tried to find this TFK - trifluoromethylketone - but I cant find a place who sells it.
I wondered if you might have an idea, maybe to use some other material...
The sample - 3 palmitoyl sn glycerol (for the calibration curve) is dissolved in chloroform.
By the way, I got a different column - Fortis H2o - our salesman said it will fit.
Thank you in advance, Adi