adulterants in HPLC 99%+ galactose

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

2 posts Page 1 of 1
Greetings all,

My first time posting. I bought a large sample of D+galactose powder rather cheaply in bulk, but it has some colorants, and possibly other contaminants. They cause a broad and quite significant fluorescence background with near-infrared Raman spectroscopy. Does anyone have a suggestion on how to quickly remove the impurities without too much expense?

Since I have a large sample and do not mind losing a bit of it, I am thinking of supersaturating the galactose in water and then removing the supernatant hoping that it would dissolve and carry out most of the contamination. I could do this a few times with distilled water, and then a final boil to remove all the water.

I am appreciative of any suggestions. BTW, we are not a chemistry facility, but a bunch of engineers. Thanks!

EDIT: Typo corrections.
You probably need to crystallize galactose it. You can also try to dissolve it in water and add either charcoal or some IE resin to take out colorants. Prep chromatography might be too expensive.
Vlad Orlovsky
HELIX Chromatography
My opinions might be bias, but I have about 1000 examples to support them. Check our website for new science and applications
www.helixchrom.com
2 posts Page 1 of 1

Who is online

In total there is 1 user online :: 0 registered, 0 hidden and 1 guest (based on users active over the past 5 minutes)
Most users ever online was 1117 on Mon Jan 31, 2022 2:50 pm

Users browsing this forum: No registered users and 1 guest

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry