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- Posts: 2
- Joined: Wed May 01, 2019 8:41 pm
I'm currently running purity and assay tests on an organic metal chelate (complexed with iron, 5 wt%). I'm using a C18 endcapped stationary phase and ACN/water mobile phase with TFA (gradient 5% --> 95% organic) combined with UV detection. However, I'm experiencing increase of area response and decrease of resolution over time. I was wondering whether this might perhaps be caused by stationary phase saturation/deterioration by iron over time?
Does anyone have experience with this problem or with analysis of organic metal chelates by RP HPLC in general?
Thank you for your response.