Can I ask the experienced members of this forum how they came to use a certain type of bracketing standard layout? There are so many options in the Empower wizard such as overall, sliding with no overlap etc. Is there one set-up that is better than others and why?

I run typically about 60-100 samples per run, 5 minutes per injection and our company have used a sliding no overlap set up for all our samples, no matter the run time or test. Its more a habit now than anything else. Some analysts say that using sliding no overlap is more accurate, others say using all standards to quantify all samples (Modified Overall) is actually the best layout but even they cant seem to back these opinions up with sound scientific reasoning. Drift is not generally an issue with our methods as the RT of the actives remain stable through analysis, bar of course instrument errors.

Can anyone give a decent reason why to use a particular bracketing standard layout?? Thanks.