-
- Posts: 181
- Joined: Tue Oct 28, 2008 9:54 pm
- Location: usa
I'm working on a substance that has two phenyl rings. I thought I could use a phenyl column (acquity CSH phenyl-hexyl, phenomenex F5) to maximize my selectivity, but all i got was unacceptable tailing.
Its a water soluble substance with a negative charge and two phenyl rings
Buffer was 15 mM and 50 mM PO4 in water @ pH 6.6 (away from any pka's)
Organic was MeOH and ACN was also tried and both gave tailing > 3
Can anyone advise on what went wrong?
Thanks,
Analytical Chemist and Adventurer
Venture Industries
Spider-Skull Island