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- Posts: 13
- Joined: Thu Sep 20, 2018 10:43 am
Critical parameters
Wavelength: 205 Nm
Injection volume: 100 Microliter
Diluent: 15% acetic acid solution
Thank you
Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.
pharm2004 wrote:
We have developed HPLC method for cleaning validation. However, area repeatability cannot be achieved. We checked the wavelength, the injector, solubility, the filter, peak shape and hlpc system. RSD % about 12% for three injections. what could be the reason for this?
Critical parameters
Wavelength: 205 Nm
Injection volume: 100 Microliter
Diluent: 15% acetic acid solution
Thank you
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