Retention time delay in a C18 column

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

4 posts Page 1 of 1
Hello,

I washed my Hplc C18 column with water for 40 min in order to get rid off the acids contamination and now I have 2 mins delay to all of my RT. How can l equilibrate my system after that? Is there a possibility to have destroyed my column? :cry:


Thank you

Barbara
Washing a C-18 column with water not destroy the column , according to my experience , but a phase collapse may occur.
That means , it requires much more time to re-equalibrate the column.

Washing with a strong solvent , such as pure methanol or acetonitrile , restores the column.

If you have 2 minutes delay for all of your peaks , there may be a pump ( flow ) problem ,please check your pump performance , by collecting the column out to a graduated cylinder against time.
I had a C-8 column that would have almost no retention after sitting with 100% water, but if I washed it with 100% methanol and let it set overnight it would then be as good as new.
The past is there to guide us into the future, not to dwell in.
Hi James ,

If you store your C-8 column in an aprotic solvent ; such as acetonitrile , life time will be longer.
Methanol seems not very suitable for short chain columns ( C-8 , C-6 , C-4 etc )because of hydrolysis.
4 posts Page 1 of 1

Who is online

In total there are 2 users online :: 1 registered, 0 hidden and 1 guest (based on users active over the past 5 minutes)
Most users ever online was 1117 on Mon Jan 31, 2022 2:50 pm

Users browsing this forum: John Guajardo and 1 guest

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry