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- Posts: 656
- Joined: Tue Jul 05, 2005 7:45 am
- Location: Denmark
I have just analysed a number of solutions prepared from the same stock, but diluted with solutions containing different amounts of NaCl (from 0 to 150 mM).
Standard RP-method, full loop injection on Waters Acquity (10 µl). Luna C18 column.
The samples were randomised during the sequence. The content result should be the same for all solutions, but there is a 100% correlation between the salt content and the result. The more salt, the lower the content of the active (about 10% difference between highest and lowest)
I see no degradation peaks in any of the samples.
If it had been adsorption, the salt should give higher results? Do you have any idea what has happened here?