100% aqueous mobile phase
Posted: Thu Feb 28, 2019 9:04 am
Dear all ,
lately my lab have received a new method for tobramycin determination, this new method don't use derivatization as USP did , its mobile phase is 100% buffer solution of pH 10 , on C18 column , and wavelength , 210 nm , so my questions if you can help me , did anyone experienced a 100% aqueous mobile phase ?!! i have read that it may cause column collapse , secondly what is your opinion in injecting tobramycin as is without derivatization ?
regarding that USP method for determination of tobramycin uses derivatization with 4-floro,2,4-dinitro benzene
lately my lab have received a new method for tobramycin determination, this new method don't use derivatization as USP did , its mobile phase is 100% buffer solution of pH 10 , on C18 column , and wavelength , 210 nm , so my questions if you can help me , did anyone experienced a 100% aqueous mobile phase ?!! i have read that it may cause column collapse , secondly what is your opinion in injecting tobramycin as is without derivatization ?
regarding that USP method for determination of tobramycin uses derivatization with 4-floro,2,4-dinitro benzene