Page 1 of 1
BUTAPHOSPHAN ESTIMATIO BY HPLC
Posted: Wed Feb 27, 2019 10:44 am
by DeviSirisha
Is it possible to determine butaphosphan by using UV-Detector?
Re: BUTAPHOSPHAN ESTIMATIO BY HPLC
Posted: Thu Feb 28, 2019 11:49 pm
by Vlad Orlovsky
Butaphosphan has no UV activity. You can try t do indirect detection which is not a very good approach, derivatization or alternative detection techniques (ELSD, RI, CAD, MS)
Re: BUTAPHOSPHAN ESTIMATIO BY HPLC
Posted: Tue Mar 05, 2019 8:46 am
by DeviSirisha
Can you suggest any alternative method
Re: BUTAPHOSPHAN ESTIMATIO BY HPLC
Posted: Tue Mar 05, 2019 5:53 pm
by Vlad Orlovsky
Do you have access to any other detection techniques - ELSD, CAD, RI, MS, nitrogen detector?
Re: BUTAPHOSPHAN ESTIMATIO BY HPLC
Posted: Mon Mar 11, 2019 9:24 am
by DeviSirisha
None of the technique is available
Re: BUTAPHOSPHAN ESTIMATIO BY HPLC
Posted: Wed Mar 13, 2019 5:12 pm
by Vlad Orlovsky
you can try indirect detection, but it is not of my favorite approaches. You can add some neutral highly UV active compound into the mobile phase and observe your compound as a negative peak.