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- Posts: 15
- Joined: Fri Aug 31, 2018 10:12 pm
I've been testing out some reagents to find a good internal standard for my samples. By "good" internal standard I just want a nice identifiable peak, since I'm also going to connect our LC to a LC-MS it would be relatively straightforward to identify which peak is my internal standard using MS spectra.
One compound that I'm testing didn't show up on the UV Vis spectrum at all, and I'm wondering if that could be related at all to its low molecular weight (~100g/mol, the compounds I target with my LC have a molecular weight of 500~1500g/mol). I'm aware that the retention time of each compound is related to how polar/nonpolar those compounds are, but was thinking if molecular weight can be related to retention time at all? (For instance, if I was using a GC instead of a LC, I think it would be possible for lighter molecules to evaporate faster and elute pretty quickly? Not sure how that would be the case with the LC. Even in this example the retention time would surely depend on more things than just molecular weight, but just curious)