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- Posts: 17
- Joined: Mon Mar 14, 2016 9:21 pm
already removed the column and there is still no flow.
any help appreciated.
Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.
hplcnewuser1 wrote:
thanks everyone. I am able to narrow it down now. I Turned off the system and when I unscrewed the thin plastic tube, from multichanel mixing valve into the pump, I saw mobile phase flow coming through.
James_Ball wrote:
If pressure build but falls again with the rhythm of the pump, then the outlet check valve is not closing
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