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Column test and retest specification
Posted: Fri Feb 01, 2019 8:50 am
by Kris_Markovs
Hi,
Can anyone give advice for column starting test and periodic retest specification?
Differences per cents from manufacturers given data of symmetry, tailing, plates ect.
Re: Column test and retest specification
Posted: Fri Feb 01, 2019 1:30 pm
by Consumer Products Guy
If a column and system passed system suitability tests, considered fine at our company.
Re: Column test and retest specification
Posted: Mon Feb 04, 2019 7:06 am
by Kris_Markovs
Till now we think the same.
But it is not correct. If in manufacturer certificate theoretical plates is 20k, and Your test result ir 2.5k, it is acceptable from general system suit. requirements but not from logic side.
For example Agilent Zorbax Rx-C8 column Performance report give these numbers:
Test-
Theoretical plates - 24307
Selectivity - 1.38
USP tailing factor - 1.06
Specifications-
Th. pl. MIN - 16000
Sel. range - 1.32-1.42
Tailing range - 0.98 - 1.25
Re: Column test and retest specification
Posted: Mon Feb 04, 2019 9:57 am
by Rndirk
I have never bothered to re-test the column specifications. Like CPG, we run SSTs to monitor the column & system performance.
Keep in mind plate count, tailing, ... do not depend on the analytical column only. The manufacturer will bring this up if you decide to confront them with your results that differ from the certificate.