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- Posts: 46
- Joined: Fri May 19, 2006 7:08 pm
I would like to ask if there is a known effect of sample temperature on the chromatogram? Let's assume for simplicity that the temperature of the column is 30 degrees C, phase 4:1 (methanol/water) and we inject this mobile phase from the vial at a temperature of 25, 20, 15, 10 degrees C. What results will we get?