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Regarding the analysis of water soluble vitamins

Posted: Thu Jan 31, 2019 4:26 am
by DeviSirisha
can anyone suggest me a method to analyse all the water soluble vitamins using rp-hplc? Is it possible to estimate vitamins using phenyl column?

Re: Regarding the analysis of water soluble vitamins

Posted: Thu Jan 31, 2019 5:20 pm
by MrGC
There you go:
https://www.sigmaaldrich.com/technical- ... n-c18.html

Information like this can be easily found via google.
Quantification is obviously possible as well if you have the respective standards of each vitamin.

Re: Regarding the analysis of water soluble vitamins

Posted: Sat Feb 02, 2019 10:41 am
by DeviSirisha
I tried several methods that are available in google. but, none of the methods gave me accurate results. so i need a rapid and precise mehod for better quantification and seperation.