Viscosity and priming

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

2 posts Page 1 of 1
(debate at work) Why are lower viscosity solvents preferred when priming the systems (after buffer was washed out). Are they somehow better at removing air bubbles?

I'm guessing the answer is simple, I just don't see it.
Possibly because the higher viscosity liquids stick to and clog up the check valves on HPLCs and UPLCs quicker than lower viscosity ones?
2 posts Page 1 of 1

Who is online

In total there are 10 users online :: 0 registered, 0 hidden and 10 guests (based on users active over the past 5 minutes)
Most users ever online was 599 on Tue Sep 18, 2018 9:27 am

Users browsing this forum: No registered users and 10 guests

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry