need help about running Lauryl Betaine on the HPLC or UPLC
Posted: Wed Dec 19, 2018 2:14 pm
by Mohamad
Hello,
I am trying to find a method to run lauryl betaine on the HPLC or UPLC.
Please if someone has any information in regards to helping me in this situation, please advise me.
thanks
Re: need help about running Lauryl Betaine on the HPLC or UPLC
Posted: Wed Dec 19, 2018 3:23 pm
by Vlad Orlovsky
You can use this method for your analysis:
https://helixchrom.com/compounds/cetylpyridinium/Your main issue is going to be tailing on C18 columns. You will need ELSD, CAD for this. You can try 200-210 nm UV but will need to replace TFA with phosphoric or sulfuric acid for better transparency and LOD/LOQ
Re: need help about running Lauryl Betaine on the HPLC or UPLC
Posted: Thu Dec 20, 2018 9:21 pm
by Mohamad
thank you Vald for the help.
but i can't see in that page any info about how to make the STD.
can you please send me this method like PDF.