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- Posts: 656
- Joined: Tue Jul 05, 2005 7:45 am
- Location: Denmark
Just wonder if this is an approach that is known to work...?
(using two pumps and a switch valve)
- Inject sample in a highly aqueous mobile phase on a C18 trap column
- Switch the valve and elute on a HILIC column with very high% acetonitrile
I assume that the trap column will contain some water that can destroy the HILIC separation? Or is it negligible amounts?
Thanks!