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estimation of eucalyptus oil

Posted: Mon Dec 03, 2018 11:46 am
by DeviSirisha
hi,
can anyone suggest me how to estimate eucalyptus oil by using c18 column in rp-hplc

Re: estimation of eucalyptus oil

Posted: Mon Dec 03, 2018 10:30 pm
by Gizmo
Tough to say without knowing your matrix but I think this paper might be a good place to start:

https://pdfs.semanticscholar.org/d3a9/b ... 16bb86.pdf

Re: estimation of eucalyptus oil

Posted: Wed Dec 05, 2018 7:40 am
by DeviSirisha
thank you for this information.
matrix is silica gel bonded to carbon atoms. can you suggest any other method

ESTIMATION OF OILS BY Rp-HPLC

Posted: Wed Dec 05, 2018 7:44 am
by DeviSirisha
can anyone suggest me a method for estimation of different types of oils like tea tree oil, eucalyptus oil, lavendin oil, camphor and menthol in a single run.
my availability is schimadzu hplc and kromasil C18 column

Re: estimation of eucalyptus oil

Posted: Wed Dec 05, 2018 5:09 pm
by JMB
There is nothing to be gained by re-inventing the wheel, so I would suggest entering the terms “HPLC & eucalyptus oil” in your favorite search engine.
Select a method that describes RP separation of multi-component essential oils.
Use this as a template for your particular situation.

Re: ESTIMATION OF OILS BY Rp-HPLC

Posted: Wed Dec 05, 2018 6:51 pm
by Consumer Products Guy
DeviSirisha wrote:
can anyone suggest me a method for estimation of different types of oils like tea tree oil, eucalyptus oil, lavendin oil, camphor and menthol in a single run.
my availability is schimadzu hplc and kromasil C18 column


First of all: you're limited severely by being restricted to HPLC and C18 column only; this is not a good way to start out any analysis - by limited equipment/technique. GC or GCMS can be useful for eucalyptol, camphor, menthol......

Re: estimation of eucalyptus oil

Posted: Thu Dec 06, 2018 8:59 am
by DeviSirisha
Thank you all for your suggestions..
At present only HPLC is available for me. I dont have GC/GCMS/LCMS or any other advanced equipment at my college. so i want to estimate oils by simple method using Rp-HPLC.

Re: estimation of eucalyptus oil

Posted: Thu Dec 06, 2018 1:50 pm
by Consumer Products Guy
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