Huge Noise

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

3 posts Page 1 of 1
Hi.
I`m currently working in some pesticides residue analysis with Waters Acquity Class H UPLC with Xevo TQD Mass Spectrometry.
I set up the system today and run some samples to plot the curve, but when i looked at the chromatogram i realised there was a huge noise until 6,50 minutes when it dissapeared. Anyone knows what its heppening here?

Water Chromatogram:
Image

Spiked Sample:
Image
Hi,

Most likely, the divert valve is set to waste after 6.7min.

It's not possible to comment if your noise is significant because we don't know anything about the method. What is the problem exactly?
And you were right my friend. It was only the divert valve.
Now just need to clean up the system. Guess i injected too much, now that damn peak its showing up everywhere. :cry:

Image
3 posts Page 1 of 1

Who is online

In total there are 9 users online :: 0 registered, 0 hidden and 9 guests (based on users active over the past 5 minutes)
Most users ever online was 599 on Tue Sep 18, 2018 9:27 am

Users browsing this forum: No registered users and 9 guests

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry