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Would a successful method validation at a wavelength on the slope prove that the method and generated data is scientifically sound?
Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.
My take on this is that the answer is "yes". Operating on a sloping part of the absorbance spectrum increases the *potential* for error if the wavelength or the spectrum shift. Proper validation should demonstrate whether or not that potential problem is an actual problem.Would a successful method validation at a wavelength on the slope prove that the method and generated data is scientifically sound?
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