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- Posts: 1
- Joined: Thu Sep 27, 2018 3:32 pm
System:
Electrochemical detector Chromsystems CLC 100
Autosampler Chromsystems CLC 220
Agilent C8 column (100x2.1)
Protocol:
Mobile phase is 150 mM NaH2PO4, 4.8 mM Citric acid, 3mM SDS, 50 uM EDTA, 11 % MeOH, 17% ACN, pH 5.6 with HCL
Electrode set at 550 mV
Flow rate is 0.4 mL/min
Sample preparation:
100 ul Serum + 150 ul ACN, 0.01M HCL
15 min on ice
Centrifuge 13200 rpm, 10 min
Discussion:
The unknown peak occur before peak of serotonin in 0.6 min. (peak of serotonin 4.2 min, unknown peak 3.6 min). Height the unknown peak the same of serotonin peak.
I have taken serum without serotonin. I have got the unknown peak again.
I changed amount of ACN (1/4).
Questions:
What am I doing incorrect? Maybe I must take another precepitation buffer...?