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- Posts: 44
- Joined: Mon Dec 18, 2017 9:09 pm
I have read in some scientific papers that:
The concentrations of the calibration curve should be evenly spaced over the chosen concentration range.
However, I am normally making the serial dilutions in this order: 100 ppb to 50ppb-25ppb-12.5ppb-6.25ppb-3.125ppb-1.56ppb-0.78ppb-0.39ppb-0.195ppb-0.0975ppb. But I realized that these data points dont have the same density. What do you think about my ranges? is this acceptable?
May I ask what do you recommend? I was thinking about having the data points like 100ppb-90ppb-80ppb-70ppb-60ppb-50ppb-40ppb-30ppb-20ppb-10-0.1ppb.
May I ask if this is acceptable? My problem with this new range is that even though they are evenly distributed but I may get a problem with finding my limit of detection at lower concentration because there are not so many points below 1 ppb whereas in the first example I have enough data points below 6.25 ppb which could help me to correctly find my limit of detection by visual inspection of the peaks.
May I ask what is your recommendations? I am really confused.
Thank you with your answers in advance
Best
Kourosh