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- Posts: 2
- Joined: Sun Sep 16, 2018 2:57 pm
I'm having issues with air bubbles in a gold system HPLC and the resulting oscillation on the UV reading.
I have looked at previous postings here and I have troubleshot the list below:
1) Not a degassing or mixing cell problem: Solvents were fully degassed and the problem does not happen when bypassing the column.
2) I have tried multiple columns: All of them show the presence of equally spaced air bubbles after the column. Even when the column has very low back pressure.
3) I have tried adding a narrow capillary right after the column and right before the UV detector entrance: The problem persists.
The UV oscillation is so bad it is killing my UV reading and resolution. I have tried changing the UV reading frequency and had no luck.
Any advice would be helpful.
bests
J. Paulino