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- Posts: 2
- Joined: Wed Sep 26, 2018 6:11 am
I'm developing a gradient HPLC method for pharmaceuticals. My last chromatographic peak appears at B ~70%. To shortn the method I'm considering to start the column equilibration directly after the last peak instead of waiting until B reaches 95%. Is this a good idea taking into account that I will run environmental samples with a high degree of impurities or is it better to implement a flushing window with B 95%?
I'm running an LC-MS/MS method in MRM mode so that there are usually no disturbing peaks orgining from impurities.