Chromatography Forum

Hi,

I am using LC450 preparative column with C18 stationary phase. Hardware part of the column design is mentioned as pressure max on the jack : 258bar and pressure max on the column tube : 100bar. Ratio is 2.58.
Currently we are applying pressure around 190bar on the jack which is almost only some 73% pressure on column tube.

Can anybody please suggest what can be the maximum pressure that i can apply on column tube or otherwise Jack pressure ? Would C18 resin (10 micron particles) withstand that much pressure?

Please help.

Virtually *any* silica-based column packing in a 10-micron particle size should hold up to 100 bar. That type of material was typical of analytical-scale columns in the '70s, and instruments in those days were rated for 300 bar or more. The possible exception would be large-pore material (40 nm or above), in which case, best to check with the manufacturer. For a prep system, the hardware will be the limitation, not the packing.