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- Posts: 301
- Joined: Tue Jul 12, 2016 4:47 pm
Post-run, i flush the A1/B1 lines in seal wash for an hour and store all lines in 50:50 Methanol until i need it again but the last 2 runs i have been getting my first system suit injection with the retention times way later than normal before it settles down perfectly stable for the remaining injections! Run time is 40 minutes and now i seem to have to try a test injection before activating my run just to "see" if retention times are normal. I always run 3 condition columns before each run, i thought that would be enough to equilibrate the system, should i add more, does anyone know what could cause this?