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- Posts: 35
- Joined: Sun Apr 08, 2018 3:17 am
I’ve been trying to find alternatives to sodium octanesulfonate as an ion pairing agent. I’ve recently tried hfba however this chemical I can only find in 50 ml bottles and is extremely stinky. I’ve also just recently tried hexafluorphosphoric acid HPF6. To make my mobile phase however and have I ended up preparing the following
40ml hpf6 into 2 l of water adjust the ph to 3.
Mobile phase a: 500ml buffer,450ml water, 50ml acetonitrile
Mobile phase b: 500ml buffer 500ml acetonitrile
My question is, is there anything wrong with HPF6 at such a high concentration especially with damaging the column and hplc/uplc? I’m adjusting the ph to within an acceptable range but I’m still worried that this may be corrosive. Does anyone have any experience with this reagent as an ionpaorong agent?