HPF6 as ion pairing agent
Posted: Mon Sep 03, 2018 7:13 pm
Hello,
I’ve been trying to find alternatives to sodium octanesulfonate as an ion pairing agent. I’ve recently tried hfba however this chemical I can only find in 50 ml bottles and is extremely stinky. I’ve also just recently tried hexafluorphosphoric acid HPF6. To make my mobile phase however and have I ended up preparing the following
40ml hpf6 into 2 l of water adjust the ph to 3.
Mobile phase a: 500ml buffer,450ml water, 50ml acetonitrile
Mobile phase b: 500ml buffer 500ml acetonitrile
My question is, is there anything wrong with HPF6 at such a high concentration especially with damaging the column and hplc/uplc? I’m adjusting the ph to within an acceptable range but I’m still worried that this may be corrosive. Does anyone have any experience with this reagent as an ionpaorong agent?
I’ve been trying to find alternatives to sodium octanesulfonate as an ion pairing agent. I’ve recently tried hfba however this chemical I can only find in 50 ml bottles and is extremely stinky. I’ve also just recently tried hexafluorphosphoric acid HPF6. To make my mobile phase however and have I ended up preparing the following
40ml hpf6 into 2 l of water adjust the ph to 3.
Mobile phase a: 500ml buffer,450ml water, 50ml acetonitrile
Mobile phase b: 500ml buffer 500ml acetonitrile
My question is, is there anything wrong with HPF6 at such a high concentration especially with damaging the column and hplc/uplc? I’m adjusting the ph to within an acceptable range but I’m still worried that this may be corrosive. Does anyone have any experience with this reagent as an ionpaorong agent?